Last edited by Nelar
Wednesday, April 29, 2020 | History

4 edition of Enhanced bioremediation of BTEX using immobilized nutrients found in the catalog.

Enhanced bioremediation of BTEX using immobilized nutrients

Enhanced bioremediation of BTEX using immobilized nutrients

field demonstration and monitoring

by

  • 323 Want to read
  • 15 Currently reading

Published by National Exposure Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency in Research Triangle Park, NC .
Written in English

    Subjects:
  • Groundwater -- Pollution,
  • Hazardous substances -- Biodegradation,
  • Bioremediation,
  • Remediation wells

  • Edition Notes

    Statementby Robert C. Borden ... [et al.].
    ContributionsBorden, Robert C., National Exposure Research Laboratory (U.S.), North Carolina State University. Dept. of Civil Engineering.
    The Physical Object
    FormatMicroform
    Pagination1 v. (various pagings)
    ID Numbers
    Open LibraryOL17824689M
    OCLC/WorldCa39218819

    Investigators cannot fully understand the details of whether and how bioremediation is occurring at a site. The goal in evaluating in situ bioremediation is to assess whether the weight of evidence from tests such as those described above documents a convincing case for successful bioremediation.   Bioremediation is an option that offers the possibility to destroy or render harmless various con­taminants using natural biological activity. As such, it uses relatively low-cost, low-technology tech­niques, which generally have a high public acceptance and can often be carried out on site. EOS Products are formulated to optimize in situ remediation of contaminated soil and groundwater. We provide electron donors for chlorinated solvents, electron acceptors for petroleum products, bioaugmentation cultures for low microbe populations, iron formulations to enhance anaerobic reductive dechlorination, and a variety of supplements designed to .


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Enhanced bioremediation of BTEX using immobilized nutrients Download PDF EPUB FB2

Nitrate addition enhanced the aerobic biodegradation of BTEX as in batch reac- tor experiments using ground water from the site. Incorporating sodium nitrate into the concrete briquets at to % by weight during the second and third treat- ment periods of the field experiments did not cause regulatory levels for nitrate to be exceeded.

@article{osti_, title = {Enhanced bioremediation of BTEX using immobilized nutrients: Field demonstration and monitoring}, author = {Borden, R.C. and Goin, R.T. and Kao, C.M. and Rosal, C.G.}, abstractNote = {A permeable barrier system was developed for controlling the migration of dissolved contaminant plumes in ground water.

The barrier system consisted of a. Enhanced bioremediation of BTEX using immobilized nutrients: field demonstration and monitoring Author: Robert C Borden ; National Exposure Research Laboratory (U.S.) ; North Carolina State University.

EPA//R/ December Enhanced Bioremediation of BTEX Using Immobilized Nutrients Field Demonstration and Monitoring by Robert C.

Borden, Russell Todd Goin and Chin-Ming Kao Department of Civil Engineering North Carolina State University Raleigh, NC Charlita G. Rosal Characterization and Monitoring Branch Environmental Sciences. Enhancement of in situ anaerobic biodegradation of BTEX compounds was demonstrated at a petroleum-contaminated aquifer in Seal Beach, CA.

Specifically, combined injection of nitrate and sulfate into the contaminated aquifer was used to accelerate BTEX removal as compared to remediation by natural by: Biodegradation of organic contaminants in soil is often limited by the availability of oxygen.

The injection of oxygen releasing chemicals can be a cost effective remediation option for relatively low BTEX (Benzene, Toluene, Ethlybenzene, Xylene) concentrations in. Enhanced bioremediation of BTEX contaminated groundwater in pot-scale wetlands.

Pot-scale wetlands were used to investigate the role of plants in enhancing the performance of engineered bioremediation techniques like biostimulation, bioaugmentation, and phytoremediation collectively. A novel oxygen-releasing immobilized bead (ORICB) was prepared.

The CaO 2-encapsulated oxygen-releasing bead has high oxygen release capacity. The ORICBs-column rapidly and effectively degrades BTEX at HRT of day. A high influent BTEX concentration did not markedly inhibit bioremediation. The DGGE profile indicates that the BTEX degrader Cited by: In this research, biodegradation of hexadecane as a model contaminant in solid soil using both free and immobilized Pseudomonas Aeruginosa, capable of producing biosurfactant, was investigated.

Coconut fibers in three mesh sizes were used as a cellulosic biocarrier for immobilization : Mahdiyeh Hajieghrari, Parisa Hejazi. The new phenomenon is the application of this technology to in situ groundwater -situ bioremediation refers to the use of naturalmicrobiological processes occurring in the subsurface environment to breakdown complex compounds into simpler, non-toxic compounds namely carbon dioxide and water without removal of aquifer rganisms (yeast, Author: Naraboyina Devika, A.

Rastogi. Sulfate-Enhanced Bioremediation. This case study describes using sulfate-enhanced bioremediation to stimulate anaerobic degradation of hydrocarbons in groundwater under sulfate-limiting conditions.

A former gas station in North Carolina contaminated groundwater with BTEX from a UST basin. The site is now in the median of a busy four-lane highway.

Get this from a library. Enhanced bioremediation of BTEX using immobilized nutrients--field demonstration and monitoring: project summary.

[Robert C Borden; National Exposure Research Laboratory (U.S.)]. Immobilized cells have been extensively used in the production of useful chemicals, treatment of wastewaters and bioremediation of pollution cause of its longer operating lifetime and enhanced stability and survival of the cells [7, 8].

The use of immobilized cells has been investigated as an alternative technology for environmental by: Novel Oxygen-releasing Immobilized Cell Beads for Bioremediation of BTEX-contaminated Water Article in Bioresource Technology –51. To isolate bacteria responsible for the biodegradation of naphthalene, BTEX (benzene, toluene, ethylbenzene, and o- m- and p-xylene), and aliphatic hydrocarbons in petroleum-contaminated soil, three enrichment cultures were established using soil extract as the medium supplemented with naphthalene, BTEX, or by: 8.

Margesin R, Walder G, Schinner F () Bioremediation assessment of a BTEX-contaminated soil. Acta Biotechnol 23(1)–36 CrossRef Google Scholar McNaughton LR, Dalton B, Palmer G () Sodium bicarbonate can be used as an ergogenic aid in high intensity, competitive cycle ergometry of 1 h : S. Ashok, V.

Akila, P. Ayyasamy, S. Rajakumar. In this study, we investigated the bioremediation of petrochemical wastewater containing BTEX compounds by immobilized Comamonas sp. JB cells. Three kinds of magnetic nanoparticles were evaluated as immobilization supports for strain JB.

After comparison with Fe3O4 and a-Fe2O3 nanoparticles, r-Fe2O3 nanoparticle was selected as the optimal Cited by: Article Sulfate-Enhanced bioremediation of BTEX - Case Study. Problem A former gas station in North Carolina contaminated groundwater with BTEX from a UST basin.

The site is now in the median of a busy four-lane highway. Residents within feet of. Description: Typical Enhanced Bioremediation System Enhanced bioremediation is a process in which indigenous or inoculated micro-organisms (e.g., fungi, bacteria, and other microbes) degrade (metabolize) organic contaminants found in soil and/or ground water, converting them to innocuous end products.

Nutrients, oxygen, or other amendments may be used to enhance bioremediation. >> Enhanced Bioremediation: Introduction>> The rate of bioremediation of organic contaminants by microbes is enhanced by increasing the concentration of electron acceptors and nutrients in ground water, surface water, and leachate.

Oxygen is the main electron acceptor for aerobic bioremediation. This chapter reviews the application of additives used in bioremediation of chlorinated solvents and fuels for groundwater and soil remediation.

Soluble carbon substrates are applicable to most site conditions except aquifers with very high or very low groundwater flow. Slow-release and solid substrates are intended to be long-lasting in supplying carbon for microbial growth thereby Cited by: 1.

Cunningham JA, Rahme H, Hopkins GD, Lebron C, Reinhard M. Enhanced in situ bioremediation of BTEX-contaminated groundwater by combined injection of nitrate and sulfate. Environ. Sci. Technol. 35 (8): Crossref, Medline, Google by: 7. Addition of SMC results in enhanced PAH-degrading efficiency (82%) as compared to the removal by sorption on immobilized SMC (46%).

It was observed that the addition of SMC to the contaminated medium reduced the toxicity, added enzymes, microorganisms, and nutrients for the microorganisms involved in degradation of PAHs (Lau, et al., ).Cited by: 5 Bioremediation Methods 22 Method Parameters 23 In Situ and Ex Situ Bioremediation 24 Biostimulation and Bioaugmentation of Contaminated Sites 25 Monitored Natural Attenuation 25 Soil Vapor Extraction, Air Sparging, and Bioventing 26 Use of Biosurfactants 28 Rhizosphere Bioremediation 28File Size: 1MB.

To overcome these limitations, scientists have been exploring the use of “enhanced” intrinsic bioremediation to speed site clean-up (Morin, ). This technology involves supplementing subsurface populations with nutrients and/or electron acceptors, which may be limiting in order to stimulate biodegradation of contaminants.

Industrial and agricultural activity throughout this century has led to considerable contamination of soil and groundwater resources by hazardous chemicals. The technique of bioremediation uses living organisms--usually bacteria and fungi--to remove pollutants from soil and water with minimal disturbance to these environments.

This approach, which is potentially 4/5(1). Modeling Systems and Processes in Wetlands: A Case Study of Engineered Bioremediation of BTEX-Contaminated Water in Treatment Wetlands. , DOI: /_ Sangeeta Anand, Sushil Kumar Bharti, Cited by: The goal in bioremediation is to stimulate microorganisms with nutrients and other chemicals that will enable them to destroy the contaminants.

The bioremediation systems in operation today rely on microorganisms native to the contaminated sites, encouraging them to work by supplying them with the optimum levels of nutrients and other chemicals. Applied Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is CHF (Swiss Francs).

Submitted papers should be well formatted and use good. Below is a list of substrate-specific guidance for several commonly used organic substrates for enhanced in situ anaerobic bioremediation. Protocol for Enhanced In Situ Bioremediation Using Emulsified Edible Oil Robert Borden, Solutions-IES.

Environmental Security Technology Certification Program, pp, May @article{osti_, title = {Enhanced in-situ biodegradation of petroleum hydrocarbons using passive addition of oxygen to groundwater}, author = {Smyth, D J.A. and Wilson, R D and Byerley, B T and Chapman, S W and Mackay, D M}, abstractNote = {A field trial incorporating the addition of oxygen to ground water through a passive interception system to ground water in a shallow.

Introduction to In Situ Bioremediation of Groundwater ES The design process commonly includes bench- and pilot-scale treatability studies. These tests may be performed during the feasibility study or remedial design, are used to evaluate whether the proposed bioremediation remedy will be successful,and obtain important design Size: 2MB.

A laboratory study on the bioremediation of diesel contaminated soil with the earthworm Eudrilus eugeniae (Kingberg) was conducted. 5 ml of diesel was contaminated into soils in replicates and inoculated with E.

eugeniae for 90 days. Physicochemical parameters, heavy metals and total petroleum hydrocarbons were analyzed using by: 9. (O2 + Nutrients) Air/O2/Propane present;long lag ORC(MgO 2) Inconclusive HRC(Polylactate) Inconclusive Bioaugmentation Bioremedy Pilot & retail sites (Microbes+/-Nutrients) (O 2 +MC) (TBA also) (Biobarrier) UC-Davis Pilot - in progress (O 2 +PM1) o 2 o 2 o 2 o 2 BTEX MTBE M I C R O B E S SOURCE MW.

Bioremediation is defined as the process whereby organic wastes are biologically degraded under controlled conditions to an innocuous state, or to levels below concentration limits established by regulatory authorities.

By definition, bioremediation is the use of living organisms, primarily microorganisms,File Size: KB. Advances in Vacuum Heap Bioremediation at a Fixed-Site Facility: Emissions and Bioremediation Rate. Biodegradation of BTEX Compounds in a Biofilm System under Nitrate-Reducing Conditions. Bioremediation of Waste Oil-Contaminated Gravels via Slurry Reactor Technology.

Evaluation of an in Situ Bioremediation Using Hydrogen Peroxide. Enhanced Bioremediation involves the addition of microorganisms (e.g., fungi, bacteria, and other microbes) or nutrients (e.g.

oxygen, nitrates) to the subsurface environment to accelerate the natural biodegradation process. There are four major processes, briefly described below. Gaseous Nutrient Injection.

Oversites across the United States have been identified as contaminated due to leaking underground storage tanks (LUST), according to the U.S. EPA web site. Many old fuel storage tanks have corroded and rusted with time, released their contents, and polluted groundwater with MTBE, BTEX and other petroleum hydrocarbons.

The Model Waterloo Emitter™ is an in-situ bioremedial device used to enhance the natural attenuation of contaminated groundwater. Using patented technology based on diffusion principles, it supplies oxygen or other bioenhancing amendments into an aquifer.

Oxygen release for aerobic bioremediation of BTEX and MTBE. Enhanced anaerobic bioremediation is the practice of adding hydrogen to soil and groundwater to increase the number and vitality of indigenous microorganisms performing anaerobic bioremediation.

In this process, anaerobic microbes substitute hydrogen for chlorine on chlorinated contaminant molecules, dechlorinating the compound.

LECTURE 12 BIOREMEDIATION. Bioremediation Bioremediation is the use of microorganisms to Moisture, heat, nutrients, oxygen, and pH are controlled to enhance biodegradation.

The irrigation/nutrient system is buried under the soil to pass air and nutrients either by vacuum or positive Microbial activity was enhanced by the addition of.In Situ Aquifer Bioremediation of Organics Including Cyanide and Carbon Disulfide J.A.M.

Abou-Rizk, M. E. Leavitt, and D. A. Graves No. 6 Fuel Oil Bioremediation in Fractured Bedrock A. Laszlo Kovacs and M. C. Landsman Bioremediation of Oil on Shorelines With Organic and Inorganic Nutrients P. Sveum and S. RamstadPrice: $Enhanced bioremediation of soil typically involves the percolation or injection of ground water or uncontaminated water mixed with nutrients and saturated with dissolved oxygen.

Sometimes acclimated microorganisms (bioaugmentation) and/or another oxygen source such as hydrogen peroxide are also added.